[1]周闯,张利,李普旺,等.双改性壳聚糖载药纳米胶束的制备及其性能研究[J].高分子通报,2022,(10):99-107.[doi:10.14028/j.cnki.1003-3726.2022.10.011]
 ZHOU Chuang,ZHANG Li,LI Pu-wang,et al.Preparation and Properties of Dual Modified Chitosan Based Drug-loaded Nano-micelles[J].POLYMER BULLETIN,2022,(10):99-107.[doi:10.14028/j.cnki.1003-3726.2022.10.011]
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双改性壳聚糖载药纳米胶束的制备及其性能研究()
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《高分子通报》[ISSN:1003-3726/CN:11-2051/O6]

卷:
期数:
2022年10期
页码:
99-107
栏目:
出版日期:
2022-10-20

文章信息/Info

Title:
Preparation and Properties of Dual Modified Chitosan Based Drug-loaded Nano-micelles
作者:
周闯12 张利2 李普旺1 何祖宇1 王超1 刘运浩1 宋书会1 谢木标3 杨子明1
1. 中国热带农业科学院南亚热带作物研究所, 海南省热带园艺产品采后生理与 保鲜重点实验室, 湛江 524091;
2. 中国热带农业科学院农产品加工研究所, 湛江 524001;
3. 岭南师范学院 化学化工学院, 湛江 524048
Author(s):
ZHOU Chuang12 ZHANG Li2 LI Pu-wang1 HE Zu-yu1 WANG Chao1 LIU Yun-hao1 SONG Shu-hui1 XIE Mu-biao3 YANG Zi-ming1
1. Key Laboratory of Hainan Province for Postharvest Physiology and Preservation of Tropical Horticultural Products, South Subtropical Crop Research Institute of China Academy of Tropical Agricultural Sciences, Zhanjiang 524091, China;
2. Agricultural Product Processing Research Institute of Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524001, China;
3. School of Chemistry and Chemical Enjineering, Lingnan Normal University, Zhanjiang 524048, China
关键词:
羧甲基壳聚糖两亲性纳米胶束自组装杀虫
Keywords:
Carboxymethyl chitosanAmphiphilicNano-micellesSelf-assemblyInsecticidal
DOI:
10.14028/j.cnki.1003-3726.2022.10.011
文献标志码:
A
摘要:
纳米载体由于对药物的包封和释放作用,可以有效地保护杀虫剂中药物分子免受外界条件的降解,且对目标害虫具有缓慢释放作用,在农业科学、生物科学等领域得到了大量的应用。本文通过酰基化反应将α-亚麻酸(ALA)疏水性脂肪长链和尿素(urea)亲水性基团接枝到羧甲基壳聚糖上,制备两亲性壳聚糖衍生物(U-CMCS-g-ALA),并通过红外光谱、核磁氢谱对其结构进行表征。然后,以U-CMCS-g-ALA为壁材,噻虫胺(CTD)为芯材,采用超声波自组装的方法制备了载药纳米胶束CTD@U-CMCS-g-ALA。扫描电镜显示纳米胶束呈球形,粒径分布均匀,平均粒径约为465 nm,在室温条件下具有缓释性能和pH响应性能,在弱酸和中性环境中(pH=6.5,pH=7.4)可以释放出更多的药物。最后通过人工饲料掺入法对甘蔗螟虫测试抗拒食活性,结果显示载药纳米胶束能有效抑制甘蔗螟虫的生长发育,具有一定的杀虫性能。
Abstract:
In this paper, α-linolenic acid (ALA) as the hydrophobic fatty long chain and urea as the hydrophilic group were grafted onto carboxymethyl chitosan through acylation reaction to prepare amphiphilic chitosan derivatives (U-CMCS-g-ALA), and its structure was characterized by Fourier infrared spectroscopy and nuclear magnetic resonance hydrogen spectroscopy. Then, the drug-loaded nano-micelles (CTD@U-CMCS-g-ALA) were prepared by the ultrasonic self-assembly method using U-CMCS-g-ALA as the wall material and clothianidin (CTD) as the core material. The scanning electron microscope (SEM) image of nano-micelles showed that the nano-micelles had a spherical shape with a uniform particle size distribution, with an average particle size of about 465 nm. In vitro release revealed that the CTD@U-CMCS-g-ALA nano-micelles exhibited a sustained and pH-responsive drug release property at room temperature. Finally, the antifeedant activity of CTD@U-CMCS-g-ALA nano-micelles on sugarcane borer was tested by the artificial feed incorporation method. The results showed that the drug-loaded nano-micelles could effectively inhibit the growth and development of the sugarcane borer and the antifeeding rate of CTD@U-CMCS-g-ALA on sugarcane borer wend down by more than 14.3% compared with unembedded clothianidin, which was mainly due to the encapsulation effect of the wall material U-CMCS-g-AlA that could reduce the antifeeding performance against larvae and thus showed better insecticidal activity.

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备注/Memo

备注/Memo:
收稿日期:2021-10-25;改回日期:2022-4-26。
基金项目:广东省基础与应用基础研究基金资助(2019A1515010714),海南省自然科学基金资助(520MS094,421MS078),中国热带农业科学院基本科研业务费专项资金(1630062022006,1630062022023),广东省现代农业产业技术体系创新团队建设专项资金(2019KJ140),海南省科技厅科技项目(ZDYF2021XDNY145)
作者简介:周闯(1992-),男,硕士,助理研究员,研究方向为生物质高分子材料
通讯作者:杨子明,E-mail:yangziming2004@163.com.
更新日期/Last Update: 2022-09-20